Amplification of cDNA ends based on template-switching effect and step-out PCR.
نویسندگان
چکیده
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.
منابع مشابه
Simultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCR.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 27 6 شماره
صفحات -
تاریخ انتشار 1999